BD Vacutainer® CPT™ Cell Preparation Tube with Sodium Heparin

Toubleshooting Problem:Granulocyte Contamination Greater than 10% Possible Cause Solution Centrifuge not at proper speed Adjust centrifuge speed to produce 1500-1800 RCF Centrifuge or BD Vacutainer® CPT™ tube not at room temperature (18-25*C) Allow centrifuge and BD Vacutainer® CPT™ tube to come to room temperature (18-25*C) Delay in centrifugation Centrifuge as soon as possible after obtaining blood specimen Abnormal sample with high granulocytes ratio Subsequent separation step using standard FICOLL™ HYPAQUE™ method Problem:Red blood cell contamination Possible Cause Solution BD Vacutainer® CPT™ tube or centrifuge not at room temperature (18-25*C) Allow centrifuge or contamination BD Vacutainer® CPT™ tube to come to room temperature (18-25*C) Centrifugation time too short Increase time of centrifugation (up to 30 minutes) MCHC below normal (8) Increase time of centrifugation (up to 30 minutes) Problem:Two few cells Possible Cause Solution Leucopenia Collect additional BD Vacutainer® CPT™ tube specimens as required Problem:Platelet excess Possible Cause Solution High platelet count Wash separated cells two times for 15 minutes at 100 RCF Problem:No defined or distinct mononuclear layer Possible Cause Solution Adapter incorrect size Use 16x125 mm centrifuge Tube adapter Centrifuge not calibrated correctly Have centrifuge calibrated Centrifuge speed too low Increase centrifuge speed to produce 1500-1800 RCF Centrifuge time too short Increase time of centrifugation (up to 30 minutes) Hyperlipemic sample Obtain fasting blood specimen Problem:No gel movement Possible Cause Solution Centrifuge speed too low Increase centrifuge speed to produce 1500-1800 RCF Tube or centrifuge temperature less than 18°C Warm tube or increase centrifuge setting to 18-25°C

About BD Vacutainer® CPT™ Cell Preparation Tube with Sodium Heparin Intended Use Summary & Explanation Test Principles Reagents, Supplies & Equipment Warnings & Precautions Venipuncture Technique Procedure Limitations Expected Results Performance Characteristics Troubleshooting Product Insert [PDF] References How To Order Contact Us More Molecular Diagnostics & Proteomics products

FICOLL is a trademark of Amersham Biosciences AB
HYPAQUE is a trademark of Amersham Health AS