BD Vacutainer® CPT™ Cell Preparation Tube with Sodium Citrate

Venipuncture Technique and Sample Collection

Prevention of Backflow

Since this BD Vacutainer® CPT™ Tube contains chemical additives, it is important to prevent possible backflow from the Tube with its attendant possibility of adverse reactions to the patient. To guard against backflow, the following precautions should be taken when drawing blood into the Tube:

1. Keep patient's arm in the downward position during the collection procedure.
2. Hold the Tube with the stopper uppermost.
3. Release the tourniquet as soon as the blood starts to flow into the Tube, or within 2 minutes of application.
4. Make sure the Tube contents do not touch the stopper or the end of the needle during the collection procedure.

General Instructions

NOTE: Gloves should be worn for venipuncture procedure.

1. Select the Tubes appropriate for samples desired.
2. Open needle package but do not remove needle shield. Thread needle onto holder.
3. Insert Tube into holder. LEAVE IN THIS POSITION.
4. Select site for venipuncture.
5. Apply tourniquet. Prepare venipuncture site with an appropriate antiseptic. DO NOT PALPATE VENIPUNCTURE AREA AFTER CLEANSING. Allow site to dry.
6. Remove needle shield. Perform venipuncture WITH PATIENT'S ARM IN A DOWNWARD POSITION AND TUBE STOPPER UPPERMOST (see Figure 1). This reduces the risk of backflow of any anticoagulant into the patient's circulation.
7. Push Tube onto needle, puncturing diaphragm of stopper.
8. REMOVE TOURNIQUET AS SOON AS BLOOD APPEARS IN TUBE, within 2 minutes of venipuncture. DO NOT ALLOW CONTENTS OF TUBE TO CONTACT THE STOPPER OR THE END OF THE NEEDLE DURING THE PROCEDURE.
   
   If no blood flows into the Tube or if blood ceases to flow before an adequate sample (approximately 3.0 mL as minimum blood volume for 4 mL draw and approximately 6.0 mL minimum blood volume for 8 mL draw) is collected, the following steps are suggested to complete satisfactory collection:
   1. Confirm correct position of needle cannula in vein.
   2. If a multiple sample needle is being used, remove the Tube and place a new Tube into the holder.
   3. If the second Tube does not draw, remove needle and discard in appropriate disposal device. DO NOT RESHIELD. Repeat procedure from step 1.
   
   NOTE: When using a blood collection set, a reduced draw of approximately 0.5 mL will occur on the first Tube. This reduced draw is due to the trapped air in the blood collection set tubing which enters the first Tube.
9. When first Tube has filled to its stated volume, remove it from holder.
10. Place succeeding Tubes in holder, puncturing diaphragm to initiate flow.
11. While each successive Tube is filling invert previous Tube 8 to 10 times to mix anticoagulant additive with blood. DO NOT SHAKE. Vigorous mixing can cause hemolysis.
12. As soon as last Tube is filled and mixed as above, remove needle from vein. Apply pressure to puncture site with dry, sterile gauze until bleeding stops.
13. Apply bandage, if desired.
14. After venipuncture, the top of the stopper may contain residual blood. Proper precautions should be taken when handling Tubes to avoid contact with this blood. Any needle holder that becomes contaminated with blood should be considered hazardous.
15. After collection, dispose of needle using an appropriate disposal device. DO NOT RESHIELD.