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BD ProEx™ C Reagent

BD ProEx™ C ICC   |  BD ProEx™ C IHC   |  Aberrant S-Phase Induction   |  Slide Atlas   Publications   |  Other Products   |  Order   |  Reimbursement   |  FAQs   |  Events

BD ProEx™ C for IHC

BD ProEx™ C* is a highly sensitive and specific biomarker reagent used with standard immunohistochemistry (IHC) techniques to detect the presence of Aberrant S-Phase induction in routinely collected biopsy specimens. BD ProEx™ C reagent
BD ProEx™ C is a protein-based biomarker reagent containing antibodies to MCM 2 protein and TOP2A. Increased expression of the MCM and TOP2A proteins are present in Aberrant S-Phase induction.
  •  ordering information
    • * Class 1 IHC (IVD)

    The BD ProEx™ C Process
    BD ProEx™ C slides are prepared using standard immunohistochemistry techniques performed manually or on an automated staining platform. The stained slides are evaluated qualitatively for the presence or absence of Aberrant S-phase induction.
    The BD ProEx™ C Process
    Slide Preparation: A paraffin-embedded formalin fixed tissue slide is prepared using a cytology specimen, and treated with a pretreatment buffer for target retrieval.
    Slide Staining: The slide is stained with BD ProEx™ C and detection reagents and counterstains developed for use with mouse monoclonal antibodies in immunochemistry techniques. The staining process can be performed either manually or with an automated staining platform.
    Slide Interpretation: Once stained and cover slipped, the slides are screened for the presence or absence of brown nuclear staining in cells, an indication of the presence or absence of Aberrant S-Phase induction.
     
    Detecting Aberrant S-Phase Induction with the BD ProEx™ C IHC assay In cells positive for Aberrant S-phase induction, a number of proteins are over-expressed, including MCM2 and TOP2A. BD ProEx™ C reagent contains antibodies to MCM2 and TOP2A, which when added to the slide, forms antigen:antibody complexes. IHC based detection chemistry reagents are used to visualize these complexes. A DAB based chromogen imparts a brown nuclear stain to cells that are positive for aberrant S-phase induction.
    Detecting Aberrant S-Phase Induction
    with Immunocytochemistry Assay
    S-Phase Genes
    		 Detecting Aberrant S-Phase Induction with Immunocytochemistry Assay
    Detection
     
     
    S-Phase Proteins
    BD ProEx™ C antibodies
     
    Reagents
     
    DAB
    Cell from patient with
    Aberrant S-Phase Induction
    1 Kastan M. and Bartek J. Cell-Cycle Checkpoints and Cancer. Nature, 2004. Vol. 432: 316-323.
    2 Massague J. G1 Cell Cycle Control and Cancer. Nature. 2004. Vol. 432 : 298-306.
    3 Freeman A. et all. Minichromosome maintenance proteins as biological markers of dysplasia and malignancy. Clin Cancer Res. 1999; 5: 2121-2132.
    		 
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