United States
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| Product Center | |
 Bile Esculin Azide Agar (Enterococcosel™) |
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| Cat. # | Desc. | Qty. | Unit |
| 221493 | Bile Esculin Azide Agar (Enterococcosel™) | 100 | SP |
Enterococcosel Agar, a modified esculin bile agar, is used for the rapid, selective detection and enumeration of enterococci.
| Catalog # | Description | Quantity | Unit |
| 221493 | Bile Esculin Azide Agar (Enterococcosel™) | 100 | SP |
Enterococcosel Agar, a modified esculin bile agar, is used for the rapid, selective detection and enumeration of enterococci. Enterococcosel Agar, a modified esculin bile agar, is used for the rapid, selective detection and enumeration of enterococci. Rochaix noted the value of esculin hydrolysis in the identification of enterococci.1 The enterococci were able to split esculin, but other streptococci could not. Meyer and Schonfeld incorporated bile into the esculin medium and showed that 61 of 62 enterococci were able to grow and split esculin, whereas the other streptococci could not.2 Swan used an esculin medium containing 40% bile salts and reported that a positive reaction on the bile esculin medium correlated with a serological group D precipitin reaction.3 Facklam and Moody performed a comparative study of tests used to presumptively identify group D streptococci and found that the bile-esculin test provides a reliable means of identifying group D streptococci and differentiating them from non-group D streptococci.4 According to current nomenclature, the group D antigen is considered non-specific since it is produced by the genera Enterococcus, Pediococcus and by certain streptococci.5 Isenberg et al. modified the Bile Esculin Agar formulation by reducing the bile concentration from 40 to 10 g/L and by adding sodium azide.6 This modification is supplied as BBL Enterococcosel Agar. Consult the text for a list of specimens for which this medium is recommended for primary isolation.7 Rochaix noted the value of esculin hydrolysis in the identification of enterococci.1 The enterococci were able to split esculin, but other streptococci could not. Meyer and Schonfeld incorporated bile into the esculin medium and showed that 61 of 62 enterococci were able to grow and split esculin, whereas the other streptococci could not.2 Swan used an esculin medium containing 40% bile salts and reported that a positive reaction on the bile esculin medium correlated with a serological group D precipitin reaction.3 Facklam and Moody performed a comparative study of tests used to presumptively identify group D streptococci and found that the bile-esculin test provides a reliable means of identifying group D streptococci and differentiating them from non-group D streptococci.4 According to current nomenclature, the group D antigen is considered non-specific since it is produced by the genera Enterococcus, Pediococcus and by certain streptococci.5 Isenberg et al. modified the Bile Esculin Agar formulation by reducing the bile concentration from 40 to 10 g/L and by adding sodium azide.6 This modification is supplied as BBL Enterococcosel Agar. Consult the text for a list of specimens for which this medium is recommended for primary isolation.7 See "Quality Control Procedures." Quality Control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory's standard Quality Control procedures. It is recommended that the user refer to pertinent CLSI (formerly NCCLS) guidance and CLIA regulations for appropriate Quality Control practices.
For in vitro Diagnostic Use. If excessive moisture is observed, invert the bottom over an off-set lid and allow to air dry in order to prevent formation of a seal between the top and bottom of the plate during incubation. Pathogenic microorganisms, including hepatitis viruses and Human Immunodeficiency Virus, may be present in clinical specimens. "Standard Precautions"9-12 and institutional guidelines should be followed in handling all items contaminated with blood and other body fluids. After use, prepared plates, specimen containers and other contaminated materials must be sterilized by autoclaving before discarding. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Information shown on this page is a short summary extracted from the QC/PI Manual, available as a PDF under the Related Documents section of this page.
