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MacConkey I Agar
Cat. # Desc. Qty. Unit
215197 MacConkey I Agar 20 SP
Intended Use:

This medium is used in qualitative procedures for the isolation and cultivation of gram-negative enteric microorganisms from a variety of clinical and nonclinical specimens.

Meets USP/EP/JP performance specifications, where applicable.1-3

Catalog # Description Quantity Unit
215197 MacConkey I Agar 20 SP

BBL™ Selective and Differential Prepared Plated Medium for Gram-Negative Microorganisms
MacConkey I Agar
Revision: 0707    Revision Date: 07/01/2007   
Intended Use: «

This medium is used in qualitative procedures for the isolation and cultivation of gram-negative enteric microorganisms from a variety of clinical and nonclinical specimens.

Meets USP/EP/JP performance specifications, where applicable.1-3

Intended Use: » Intended Use:

This medium is used in qualitative procedures for the isolation and cultivation of gram-negative enteric microorganisms from a variety of clinical and nonclinical specimens.

Meets USP/EP/JP performance specifications, where applicable.1-3

Product Summary: «

MacConkey I Agar is a selective and differential medium for the detection of coliform organisms and enteric pathogens.4 Bile salts and crystal violet are employed as the selective agents. Neutral red is incorporated to differentiate lactose fermenters from lactose nonfermenters.

Product Summary: » Product Summary:

MacConkey I Agar is a selective and differential medium for the detection of coliform organisms and enteric pathogens.4 Bile salts and crystal violet are employed as the selective agents. Neutral red is incorporated to differentiate lactose fermenters from lactose nonfermenters.

User Quality Control: « User Quality Control:

1. Examine plates for signs of deterioration as described under Product Deterioration.

2. Check performance by inoculating a representative sample of plates with pure cultures of stable control organisms that give known, desired reactions. The following test strains are recommended:

For clinical and other non-USP/EP/JP applications:

Test Strain Expected Results
Escherichia coli
ATCC™ 25922

Salmonella choleraesuis subsp.
choleraesuis serotype Typhimurium
ATCC 14028

Enterococcus faecalis
ATCC 29212		 Growth, pink colonies


Growth, colorless colonies



Inhibition (partial)

For USP/EP/JP applications*:

Test Strain Inoculum Incubation Expected Results
Escherichia coli
ATCC 8739		 <100 colony-forming
units (CFU)		 30–35°C for
18–72 h		 Growth comparable to
previously accepted lot of medium

*For USP/EP/JP applications, inoculum level and incubation conditions are prescribed.


Reagents: « Reagents:
Formula:
  Approximate Formula* Per Liter Purified Water
  Pancreatic Digest of Gelatin  17.0   g
  Peptones (meat and casein)  3.0   g
  Lactose  10.0   g
  Bile Salts  1.5   g
  Sodium Chloride  5.0   g
  Neutral Red  0.03   g
  Crystal Violet  0.001   g
  Agar  13.5   g
  *Adjusted and/or supplemented as required to meet performance criteria.

Warnings and Precautions: « Warnings and Precautions:

For in vitro Diagnostic Use.

If excessive moisture is observed, invert the bottom over an off-set lid and allow to air dry in order to prevent formation of a seal between the top and bottom of the plate during incubation.



Information shown on this page is a short summary extracted from the Package Insert, available as a PDF under the Related Documents section of this page.