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Hycheck™ Yeasts and Molds



Cat. # Description Qty. Unit
290006 Hycheck™ Yeasts and Molds
(Tryptic Soy Agar and Rose Bengal Chloramphenicol Agar)
10 units SP

Intended Use:

Hycheck™ is a hygiene contact slide used to assess the microbiological contamination of surfaces or fluids.


Catalog # Description Quantity Unit
290006 Hycheck™ Yeasts and Molds
(Tryptic Soy Agar and Rose Bengal Chloramphenicol Agar)
10 units SP

Difco™ Hycheck™ The hygiene contact slide with the unique hinge for monitoring microbial contamination on surfaces or in liquids.
Revision: 0802     Revision Date: 08/01/2002    

Intended Use:

Hycheck™ is a hygiene contact slide used to assess the microbiological contamination of surfaces or fluids.



Product Summary:

Monitoring the microbial flora of environmental surfaces, walls, ceilings and equipment is an important stage in achieving good manufacturing practices in factories handling foods, cosmetics or pharmaceuticals.1-3 To maintain good hygiene standards in hotels and restaurant kitchens, microbiological contamination must also be monitored.4 Methods to monitor the environmental flora have been described using either swabbing techniques 5 or contact plates.6 Contact slides were created to monitor the microbial flora of liquids (e.g. urine, milk) and equipment surfaces in the clinical and food industries.1 Contact slides are statistically comparable to swab and contact plates for surface sampling.1 The Hycheck contact slides were developed for the testing of fluids and surfaces for microbial cleanliness.

Hycheck is a double-sided, hinged plastic paddle containing two agar surfaces. The agar surface extends above the paddle allowing for contact with test surfaces. The hinged paddle allows the agar surface to be easily held against each test area during sampling. The surface area of the paddle is clearly divided into seven units of one centimeter each to allow direct counting of microbial density per unit area.

The Hycheck range of hygiene contact slides consists of seven media combinations designed to meet the various needs for monitoring different types of microbial contamination.



User Quality Control:



Reagents:

Formulae:

D/E Neutralizing Agar
  Approximate Formula* Per Liter Purified Water
  Pancreatic Digest of Casein  5.0   g
  Yeast Extract  2.5   g
  Dextrose  10.0   g
  Sodium Thioglycollate  1.0   g
  Sodium Thiosulfate  1.0   g
  Sodium Bisulfite  2.5   g
  Lecithin  7.0   g
  Polysorbate 80  5.0   g
  Bromcresol Purple  0.02   g
  Agar   15.0   g
 
Tryptic Soy Agar
  Approximate Formula* Per Liter Purified Water
  Pancreatic Digest of Casein  15.0   g
  Papaic Digest of Soybean Meal  5.0   g
  Sodium Chloride  5.0   g
  Agar  15.0   g
 
Violet Red Bile Glucose Agar
  Approximate Formula* Per Liter Purified Water
  Yeast Extract  3.0   g
  Peptone  7.0   g
  Dextrose  10.0   g
  Sodium Chloride  5.0   g
  Bile Salts No. 3  1.5   g
  Neutral Red   0.03   g
  Crystal Violet  0.002   g
  Agar  15.0   g
 
Plate Count Agar (PCA)
  Approximate Formula* Per Liter Purified Water
  Pancreatic Digest of Casein  5.0   g
  Yeast Extract  2.5   g
  Dextrose  1.0   g
  Agar  15.0   g
 
Plate Count Agar (PCA) with TTC contains 0.1 g/L in addition to the above ingredients.
 
Rose Bengal Chloramphenicol Agar (RBCA)
  Approximate Formula* Per Liter Purified Water
  Papaic Digest of Soybean Meal  5.0   g
  Dextrose  10.0   g
  Monopotassium Phosphate  1.0   g
  Magnesium Sulfate  0.5   g
  Rose Bengal  0.05   g
  Agar  15.0   g
  Chloramphenicol  0.05   g
  *Adjusted and/or supplemented as required to meet performance criteria.


Warnings and Precautions:

For Laboratory Use



Information shown on this page is a short summary extracted from the Package Insert, available as a PDF under the Related Documents section of this page.